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Katolog > Science > Biology > Biochemistry and Molecular Biology > Methods and Techniques

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    Links:

    Comparative Genomic Hybridization
http://amba.charite.de/cgh/

    Comparative Genomic Hybridization

    CGH is a molecular cytogenetic method of screening a tumor for genetic changes. CGH protocols, images, publications, events and links maintained by the University Hospital Charité, Humboldt-University of Berlin, Germany.
    amba.charite.de/cgh
    BBHelp
http://www.uct.ac.za/depts/mmi/bbhelp/intro.html

    BBHelp

    Collection of molecular biology techniques derived from help files of Dr. Harold Zappe at the Department of Medical Microbiology, University of Cape Town, South Africa.
    www.uct.ac.za/depts/mmi/bbhelp/intro.html
    Basic Principles of Gene Cloning, Sequencing, & Analysis
http://www.geocities.com:80/SiliconValley/5504/biochem.html

    Basic Principles of Gene Cloning, Sequencing, & Analysis

    Description of methods behind gene cloning, genetic engineering, DNA recombination and isolation, including discussion of restriction enzymes and ligase.
    www.geocities.com:80/SiliconValley/5504/biochem.html
    Comet Assay Interest Group
http://cometassay.com

    Comet Assay Interest Group

    Forum for exchange of views on the single cell gel electrophoresis system, capable of detecting single- and double-strand breaks, oxidative DNA base damage, and DNA, protein and drug crosslinking. Includes related links.
    cometassay.com
    SciWeb Protocols and Reagents
http://sciweb.com/features/protocols.cfm

    SciWeb Protocols and Reagents

    Directory of molecular biology protocol links.
    sciweb.com/features/protocols.cfm
    MolBio-Spot
http://www.geocities.com/richyuhk/

    MolBio-Spot

    Richard Man-kit Yu's site where you can acquire various molecular biology techniques.
    www.geocities.com/richyuhk
    Assay of Protein Concentration
http://www-class.unl.edu/biochem/protein_assay/

    Assay of Protein Concentration

    Different methods of determining protein concentration, as a resource for researchers and students in biochemistry and molecular biology laboratories from University of Nebraska, Lincoln, NE.
    www-class.unl.edu/biochem/protein_assay
    Molecular Probes, Inc.
http://www.probes.com/resources/

    Molecular Probes, Inc.

    Handbook of fluorescence applications, bibliography, data tables and calculators for molecular biology, information on courses and workshops from supplier in Eugene, OR and Leiden, Netherlands.
    www.probes.com/resources
    Practical Molecular Biology
http://molbiol.ru/eng/

    Practical Molecular Biology

    Methods, information, programs and BioMail for molecular biologists, as joint venture between Germany and Russia.
    molbiol.ru/eng
    Molecular Cloning Manual
http://www.molecularcloning.com/

    Molecular Cloning Manual

    Subscription only source of laboratory techniques in DNA and protein science, to complement the hard copy, giving scientists the benefit of both media from CSHL, NY.
    www.molecularcloning.com
    Molbiol.net
http://www.molbiol.net/

    Molbiol.net

    Resource for molecular biologists on methods, with feedback for user-directed evolution and links to commercial suppliers from Jonathan D Rees, Oxford, UK.
    www.molbiol.net
    Biomedpage
http://www.biomedpage.de/indexe.html

    Biomedpage

    Collection of laboratory protocols, applications and links to publications in biomedicine and molecular biology from author in Germany.
    www.biomedpage.de/indexe.html
    Owczarzy, Richard
http://www.owczarzy.net/

    Owczarzy, Richard

    Online calculations of nucleic acid extinction coefficients, melting temperatures, and links to biophysics databases. Includes thesis, publications and contacts for commercial scientist in Coralville, Iowa.
    www.owczarzy.net
    Biopolymer Calculator
http://paris.chem.yale.edu/extinct.html

    Biopolymer Calculator

    Calculates molecular weight, composition and concentration of nucleic acids and proteins, based on provided sequence, dilution and absorbance measurement. Also melting temperatures for nucleic acids. Maintained at Schepartz Laboratory of Chemical Biology
    paris.chem.yale.edu/extinct.html
    Molecular Biology Protocols
http://micro.nwfsc.noaa.gov/protocols/

    Molecular Biology Protocols

    Protocol collection, with interactive forum for over ten topics, covering DNA, PCR, RNA and proteins techniques and links to online journals and bioinformatics resources, from the Northwest Fisheries Science Center Microbial Pathogenesis Lab, Seattle, WA.
    micro.nwfsc.noaa.gov/protocols
    BioProtocol
http://bioprotocol.bio.com/protocolstools/

    BioProtocol

    Internet project initiated in early 1992 as BRNI, bio.com includes PCR, hybridization and microarray protocols.
    bioprotocol.bio.com/protocolstools
    Home Page of Andy Vierstraete
http://allserv.ugent.be/~avierstr/

    Home Page of Andy Vierstraete

    The use of DNA techniques in phylogenesis. Explanations, protocols and animations from Andy Vierstraete at the University of Ghent, Belgium.
    allserv.ugent.be/~avierstr
    Surgery Research
http://surgeryresearch.com/

    Surgery Research

    Collection of DNA microarray technology protocols, primarily for resident students at Stanford.
    surgeryresearch.com
    Beginning Laboratory Manual
http://www.research.umbc.edu/~jwolf/method1.html

    Beginning Laboratory Manual

    Teaching manual to support the applied biotechnology programs at the University of Maryland, Baltimore.
    www.research.umbc.edu/~jwolf/method1.html
    Recombinant DNA
http://web.mit.edu/esgbio/www/rdna/rdnadir.html

    Recombinant DNA

    Preparing probe, cloning into plasmids, complementarity and hybridization, gel electrophoresis, transfer to solid support and blotting variants, blocking, target detection PCR problems from chapter in MIT hypertext handbook.
    web.mit.edu/esgbio/www/rdna/rdnadir.html
    Tronolab Website
http://www.tronolab.com/

    Tronolab Website

    Collection of protocols, maps and sequences regarding manipulation of lentiviral vectors, siRNA and transgenic animals. Applications in HIV, stem cell and gene therapy research at University of Geneva.
    www.tronolab.com
    ECR Browser
http://ecrbrowser.dcode.org/

    ECR Browser

    Multiple alignment and dynamic evolutionary conservation profile of several vertebrate genomes including human, mouse, rat, chicken, frog, and three fishes, developed at LLNL, Livermore, California.
    ecrbrowser.dcode.org
    Hirsh Lab Gel Documentation System
http://www.virginia.edu/biology/Fac/hirsh_gel/gel/

    Hirsh Lab Gel Documentation System

    Description of an agarose gel set-up. Includes instructions for camera operation with a script, with computer side image analysis from the University of Virginia, Charlottesville.
    www.virginia.edu/biology/Fac/hirsh_gel/gel
    Protocols
http://www.uga.edu/srel/DNA_Lab/protocols.htm

    Protocols

    Microsatellites and others techniques from the Savana River Ecology Laboratory, University of Georgia.
    www.uga.edu/srel/DNA_Lab/protocols.htm
    SNP Scoring
http://las.perkinelmer.com/catalog/Category.aspx?CategoryName=SNP+Scoring

    SNP Scoring

    Overview of genotyping, detection and quantifying single nucleotide polymorphism, including the application of template directed dye terminator incorporation assay. Offers database and links to other resources from PerkinElmer Life Sciences, Boston, MA.
    las.perkinelmer.com/../Category.aspx?CategoryName=SNP+Scoring

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